Figure 3.

Specific silencing on mutant allele and protein is maintained over time

(A) Semi-quantitative Dnm2 and Atpase RT-PCR products and quantification of Dnm2 expression normalized to Atpase (HTZ n ≥ 10; WT n = 12). (B) EcoNI digestion profile of Dnm2 PCR products and quantification of the mutant/WT, mutant/Atpase, and WT/Atpase ratios (HTZ and WT n = 11). (C) DNM2 western blot and quantification of signal by densitometry. GAPDH was used as loading control (HTZ and WT n = 8). In scatterplots (A–C), the bars are mean values and error bars indicate SEM. ∗∗∗p < 0.001, ∗∗p < 0.01, and ∗p < 0.1 two-tailed using a Mann-Whitney U test comparison. HTZ, heterozygous; Mut, mutant; WT, wild type.