Fig. 2.

Fenofibrate reduced cisplatin-induced mitochondrial damage through the suppression of mitochondrial Puma/caspase-9 pathways in mProx cells.

mProx cells were treated for 24 h with cisplatin (25 μM) in the presence or absence of fenofibrate (50 μM). Whole cell lysates and cytosolic and mitochondrial fractions were prepared using methods described in the Method's section. The amounts of mitochondrial Puma (A), cleaved caspase-9 (B), JNK and phosphorylated JNK (p-JNK) (C), 14-3-3 and phosphorylated 14-3-3 (p-14-3-3) (D), β-actin and Cox IV were measured by immunoblot analyses. Each protein amount was normalized to the indicated antigen levels. A representative blot is shown in the upper panel (A-D). Results are expressed as the mean ± SD of a representative experiment (n = 3 for each group). **P < 0.01, significantly different from cells incubated under the indicated conditions, according to 1-way ANOVA with Scheffe's (A-D) post hoc comparisons.