Fig. 3.

Fenofibrate reduced cisplatin-induced caspase-8 activation through the suppression of MAPK, NFkB and death receptor pathways in mProx cells.

mProx cells were treated for 24 h with cisplatin (25 μM) in the presence or absence of fenofibrate (50 μM). Whole cell lysates were prepared for immunoblot analysis. The amounts of p38 and phosphorylated p38 (p-p38) (A), NFkB and phosphorylated NFkB (p-NFkB) (B), and ERK and phosphorylated ERK (p-ERK) (C) were measured by immunoblot analyses. Each phosphorylated protein amount was normalized to the levels of the corresponding protein. A representative blot is shown in the upper panel (A-C). The levels of TNF-α mRNA (D) were measured by real-time PCR assay and normalized to the levels of β2-microglobulin mRNA. The average level of each mRNA in untreated cells was set to 1.0. Results are expressed as the mean ± SD of a representative experiment (n = 3 for each group). *P < 0.05, **P < 0.01, significantly different from cells incubated under the indicated conditions, according to 1-way ANOVA with Scheffe's (A, C, D) or Fisher's (B) post hoc comparisons.