Table 4.
Amplification strategies | Target | LOD | Time (min) | Portability | Ref |
---|---|---|---|---|---|
RPA | N gene | 30 copies reaction−1 | 30 | Portable | [55] |
Ligation-dependent isothermal reaction | RNA-dependent RNA polymerase (RdRp) gene | 1 aM | 30 ~ 50 | Nonportable | [57] |
CRISPR-Cas13 amplification | N gene | 80 copies reaction−1 | 20 | Nonportable | [58] |
PLA | N protein | 0.0309 ng mL−1 | 120 | Nonportable | [59] |
Thermoswimming signal amplification | S protein | 26,000 aM | 15 | Nonportable | [60] |
PIFE | N protein | 50 ng mL−1 | 2 | Portable | [62] |