4. Results of the main analysis (simultaneous analysis of all tests against FISH), with results of the sensitivity analysis (separate analyses for each test with four or more studies) using FISH as the reference standard.
| Test | Number of studies | Main analysis | Sensitivity analysis | ||
| Sensitivity (95% CrI) | Specificity (95% CrI) | Sensitivity (95% CrI) | Specificity (95% CrI) | ||
| CISH | 1 | 1.00 (0.84 to 1.00) | 0.92 (0.33 to 1.00) | Insufficient studies | |
| PCR‐based LOH | 15 | 0.94 (0.83 to 0.98) | 0.94 (0.87 to 0.98) | 0.94 (0.83 to 0.98) | 0.94 (0.87 to 0.98) |
| Real‐time PCR | 2 | 0.81 (0.20 to 0.99) | 1.00 (0.95 to 1.00) | Insufficient studies | |
| MLPA | 2 | 0.96 (0.44 to 1.00) | 0.68 (0.20 to 0.95) | Insufficient studies | |
| CGH | 4 | 0.95 (0.59 to 1.00) | 0.99 (0.90 to 1.00) | 0.90 (0.64 to 0.99) | 0.99 (0.91 to 1.00) |
| aCGH | 3 | 1.00 (0.89 to 1.00) | 0.91 (0.55 to 0.99) | Insufficient studies | |
| SNP array | 6 | 0.90 (0.57 to 0.99) | 0.97 (0.84 to 1.00) | 0.90 (0.57 to 0.99) | 0.97 (0.84 to 1.00) |
| NGS | 6 | 0.94 (0.75 to 0.99) | 1.00 (0.99 to 1.00) | 0.93 (0.83 to 0.98) | 1.00 (0.99 to 1.00) |
| MS | 1 | 1.00 (0.60 to 1.00) | 1.00 (0.70 to 1.00) | Insufficient studies | |
| NanoString | 1 | 0.85 (0.11 to 1.00) | 0.80 (0.10 to 1.00) | Insufficient studies | |
aCGH: array comparative genomic hybridisation; CGH: comparative genomic hybridisation; CISH: chromogenic in situ hybridisation; CrI: credible interval; FISH: fluorescence in situ hybridisation; LOH: loss of heterozygosity; MLPA: multiplex‐ligation‐dependent probe amplification; MS; mass spectrometry; NGS: next‐generation sequencing; PCR: polymerase chain reaction; SNP: single nucleotide polymorphism.