Table 1.

Summary of study design and single-cell RNA sequencing platforms in various human cohort studies of AD. PMID: PubMed id

Study	PMID	Platform	Study design	Note
Grubman et al. 2019 [41]	31768052	10x isolated single-nuclei RNA sequencing	6 AD, 6 controls from tissue repository
Mathys et al. 2019 [8]	31042697	10x isolated single-nuclei RNA sequencing	24 AD pathology, 24 no pathology from tissue repository
Alsema et al. 2020 [25]	33192286	10x single-cell RNA sequencing and Smart-seq2	FACS sorted microglia from 27 autopsy samples within 6 h after death	10x scRNA-seq and Smart-seq2 showed some differences, mainly small clusters suspectedly due to plate-based protocols in Smart-seq2.
Lau et al. 2020 [42]	32989152	10x isolated single-nuclei RNA sequencing	12 AD and 8 control from tissue repository	Male and female ratio were balanced in AD and control
Nguyen et al. 2020 [43]	32840654	10x isolated single-nuclei RNA sequencing	15 AD from tissue repository	Samples were selected with varying APOE genotypes and pathologies, but matched for age and sex.
Gerrits et al. 2021 [44]	33609158	10x isolated single-nuclei RNA sequencing	10 AD	10 AD samples representing Braak stages 0, 2 and 6, all APOE ε3/ε3 genotypes
Morabito et al. 2021 [45]	34239132	10x isolated single-nuclei RNA sequencing and ATAC sequencing	12 AD prefrontal cortex (PFC), 8 control PFC
Olah et al. 2020 [7]	33257666	10x single-cell RNA sequencing	FACS sorted microglia from 10 AD, 4 Mild Cognitive Impairment and 3 temporal lobe epilepsy samples in DLPFC