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Sequences of primers and probe for real-time RT-RAA assays for ALV-J.
| Primer | Sequence (5’-3’) | Location |
|---|---|---|
| JF-Primer | CAACAAGCAAGAAAGACCCGGAGAAGACAC | 5363-5392 |
| JR-Primer | CACACGTTTCCTGGTTGTTGCAATAGATG | 5482-5510 |
| JP-Probe | TTCTTTCAAATGATACTTGTGTGCGTGGTTAT/i6FAMdT/ /THF/ /iBHQ1dT/TTCCGTTGTCCCAGG[C3-spacer] | 5419-5468 |
Abbreviations: ALV-J, J Avian leukosis virus; RT-RAA, reverse-transcription recombinase-aided amplification assay.
