REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides,and recombinant proteins
DMSO	Sigma-Aldrich	Cat#D8418
PEG 3350	Merck	Cat#1546547
KI	Merck	Cat#221945
LB media	VWR Life Science	Cat#J104
LB Agar	Merck	Cat#L3027
XL1-Blue cells	Agilent	Cat#200150
IPTG	VWR Life Science	Cat#0487-100G
PMSF	Gold Biotechnology	Cat#P-470-25
Protease inhibitor cocktail	Roche	Cat#11873580001
IANBD amide	Thermo Fisher Scientific	Cat#D2004
DTT	Gold Biotechnology	Cat#DTT25
TCEP	Sigma-Aldrich	Cat#C4706
Bradford Assay Reagent	Bio-Rad	Cat#500-0006
Acrylamide	Merck	Cat#110784
Na2S2O3.5H2O	Merck	Cat#1615107
Co2+ affinity resin	Takara Bio	Cat#635504
Ni-NTA affinity resin	QIAGEN	Cat#30230
Ludox	Sigma-Aldrich	Cat#420832
NATA	Merck	Cat#A6501
Imidazole	VWR Lifescience	Cat#97064-626
Critical commercial assays
PCR Kit	Merck	Cat#71086-3
Miniprep Kit	QIAGEN	Cat#27106
Software and algorithms
GC content and Tm calculation	Integrated DNA Technologies	https://www.idtdna.com/pages
DNA secondary structure predictor	Integrated DNA Technologies	https://eu.idtdna.com/calc/analyzer
Confirmation of DNA sequence	Eurofins	https://www.eurofins.com
OriginPro 8	Origin Lab	https://www.originlab.com/demodownload.aspx
Decay Analysis version 2018	Smith et al. (2017)	https://doi.org/10.7488/ds/2093
Matlab 2017a	MathWorks	https://www.mathworks.com/products/matlab.html
Other
Quick guide to different kinds of buffer solution at different pH.	Sigma-Aldrich	https://www.sigmaaldrich.com/IN/en/technical-documents/protocol/protein-biology/protein-concentration-and-buffer-exchange/buffer-reference-center
Quick guide to salt selection for buffer preparation at different pH.	Sigma-Aldrich	https://www.sigmaaldrich.com/IN/en/technical-documents/technical-article/protein-biology/protein-concentration-and-buffer-exchange/salt-selection-and-buffer-preparation
PD10 columns	Cytiva	Cat#GE 17-0851-01
PCR grade water	Sigma-Aldrich	Cat#W4502
Syringes	Merck	Cat#Z683531
Syringe filter (0.22 μM)	Merck	Cat#SLGV033RS
Filter unit (0.22 μM)	Merck	Cat#S2GPU02RE
Fernbach culture flask	Corning	Cat#4423-2XL
Filtration (Amicon) concentrator	Merck	Cat#UFC903024 Cat#UFC901024
Autoclave tape	Sigma-Aldrich	Cat#A2549
Amber glass vials	Merck	Cat#854172
Amber eppendorf tubes	Tarsons	Cat#500013
Primer resource	Integrated DNA Technologies	https://www.idtdna.com/pages
SDS-PAGE Protein Standard	Bio-Rad	Cat#1610374
Ultracentrifuge tube	Thermo Scientific	Cat#314460
Quartz Cuvette	Hellma Analytics	Cat#104-10-40-QS
Shaker incubator	Incubation and cell growth	Scigenics Biotech (Orbitek LT)
Big volume shaker	Incubation, cell growth, and protein expression	Scigenics Biotech (LE4676-AH)
Rotator	Mixing samples	Labnet H5500
Sonicator	Cell lysis	Hielscher (UP100H)
Sonicator probe	Cell lysis	Sonotrode MS7
Homogenizer/ Microfluidizer	Cell lysis or homogenization	Microfluidics LM-20
Ultracentrifuge	Separation	Thermofisher Scientific (Sorvall wx+)
Ultracentrifuge rotor	Separation	Thermo Fisher Scientific (T647.5)
Refrigerated table-top centrifuge	Spinning down cells and for concentrating proteins	Thermo Scientific Sorvall ST4 Plus (75009911)
Refrigerated table-top centrifuge rotor	Spinning down cells	TX-1000 Swinging Bucket Rotor (75003017)
Refrigerated table-top centrifuge rotor	Concentrating proteins	Fiberlite F15-6X 100Y Fixed angle rotor (75003698)
Microvolume spectrophotometer	Protein quantification	DeNovix (DS-11+)
SEC	Protein purification	Cytiva (AktaPure 25M)
Spectrofluorometer∗	Fluorescence measurements	Hitachi F7000
NanoLED#	Light source for lifetime measurements	HORIBA (NanoLED-280; NanoLED-295; NanoLED-460)