REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-GPT2	Proteintech	16757-1-AP; RRID:AB_2112098
Rabbit polyclonal anti-Akt	Cell Signaling	#9272; RRID:AB_329827
Rabbit polyclonal anti-Phospho-Akt (Ser473)	Cell Signaling	#9271;RRID:AB_329825
Rabbit polyclonal anti-p70 S6 Kinase	Cell Signaling	#9202; RRID:AB_331676
Rabbit polyclonal anti-Phospho-p70 S6 Kinase (Thr389)	Cell Signaling	#9205; RRID:AB_330944
Mouse monoclonal anti-Myosin (Skeletal, Slow)	Sigma-Aldrich	M8421;RRID:AB_477248
Mouse monoclonal anti-Skeletal Myosin (FAST)	Sigma-Aldrich	M4276;RRID:AB_477190
Mouse monoclonal anti-Myosin Heavy Chain Type IIA	DSHB	SC-71; RRID:AB_2147165
Mouse monoclonal anti-Myosin Heavy Chain Type IIB	DSHB	BF-F3; RRID:AB_2266724
Goat anti-Mouse IgG (H + L) Alexa Fluor 488	Invitrogen™	A-11001; RRID:AB_2534069
Goat anti-Mouse IgM (Heavy chain) Alexa Fluor 555	Invitrogen™	A21426; RRID:AB_2535847
Rabbit polyclonal anti-Laminin	Abcam	Ab11575; RRID:AB_298179
Rabbit polyclonal anti-GAPDH	Elabscience	E-AB-20059; RRID:AB_2905551
Mouse monoclonal Anti-Flag	Sigma-Aldrich	F3165; RRID:AB_259529
Anti-Thyroid hormone receptor antibody (C3)-Chip Grade	ABCAM	ab-2743; RRID:AB_2043044
Anti-Thyroid hormone receptor beta antibody-Chip Grade	ABCAM	ab-5622; RRID:AB_304991
Chemicals, peptides, and recombinant proteins
Dulbecco'’s Modified Eagle Medium (DMEM)	Gibco	12491-015
Fetal Bovine Serum (FBS)	Microgem	S1810-500
Sodium pyruvate	Sigma-Aldrich	S8636
Alanine	Sigma-Aldrich	A7627-1G
Glutamine	Sigma-Aldrich	G7513
D-GLUCOSE (U-13C6, 99%)	Cambridge Isotope Laboratories, Inc.	CLM-1396
L-GLUTAMINE (AMIDE-15N, 98%+)	Cambridge Isotope Laboratories, Inc.	NLM-557
L-GLUTAMINE-13C5 99%	Cambridge Isotope Laboratories, Inc.	CLM-1822-H
Goat Serum	Sigma-Aldrich	G 9023
Tetracycline-free Fetal Bovine Serum (Tet free FBS)	Clontech	631106
Doxycycline	Sigma-Aldrich	D9891
Penicillin/Streptomycin	Gibco	15140-122
Matrigel	Corning	Cat# 354248
MCDB	Sigma	Cat# M6770
Ultroser	Pall	Cat# 15950-017
Passive Lysis Buffer	Promega	E194A
Lipofectamine 2000	Invitrogen	11668-019
Immobilon Western Chemiluminescent HPR Substrate	Millipore	WBKLS0500
Protein G Plus/Protein A Agarose Suspension	Calbiochem	IP05
Trizol Reagent	Gibco	15596018
Collagenase A	Roche	11088793001
Dispase II	Roche	#04942078001
Bis-acrylamide	Sigma-Aldrich	#M1533
TEMED	Sigma-Aldrich	#M1533
APS	Sigma-Aldrich	#A3678
Eukitt® mounting medium	Bio Optica	09-00250
3,3′,5-Triiodo-L-thyronine sodium salt (T3)	Sigma-Aldrich	T6397-1G
L-Thyroxine sodium salt pentahydrate (T4)	Sigma-Aldrich	T2501
α-Ketoglutaric acid sodium salt (AKG)	Sigma-Aldrich	K1875
AIN-93G Modified + Gln diet	Envigo	223741
MitoTracker™ Red CMXRos	Invitrogen	M7512
Critical commercial assays
Immobilon Western Chemiluminescent HPR Substrate	Millipore	WBKLS0500
SYBR green master mix	Biorad	172-5121
SuperScript™ VILO™ Master Mix	Thermo Fisher	11755500
Alanine Transaminase Activity Assay kit	Abcam	ab105134
Dual-Luciferase Reporter Assay System	Promega	E1910
Deposited data
Original western blot images	This paper	Mendeley Data: https://doi.org/10.17632/jfhwj4d439.1
Experimental models: Cell lines
C2C12	ATCC	CRL-1772™
C2C12 TET ON D2	Laboratory of Monica Dentice
(Sagliocchi et al., 2019)	N/A
Primary sorted satellite cells D2WT	This paper	N/A
Primary sorted satellite cells D2KO	This paper	N/A
Experimental models: Organisms/strains
C57BL/6J	Jackson Laboratory	Stock No:000664
Gpt2tm1a(KOMP)Wtsi (GPT2KO+/-)	UC Davis, MMRRC	MGI:4363039
TRaKO and TRbKO mice	Laboratory of G. Williams, Imperial College (originally generated by J.Samarut, UMR)	N/A
Mouse Tg:Pax7-nGFP D2WT	(Sambasivan et al., 2009)	MGI:5308730
Mouse Tg:Pax7-nGFP D2KO	Laboratory of Domenico Salvatore	N/A
Oligonucleotides
Individual Oligonucleotides Listed in Table S2	Eurofins	N/A
Recombinant DNA
pGL3-GPT2 promoter-LUC	This paper	N/A
TR-α FLAG	Zavacki et al., 1996	N/A
TRPV	Zavacki et al., 1993	N/A
Software and algorithms
Cell∗F Olympus Imaging Software	Olympus	www.soft-imaging.net
ImageJ 1.45s	National Institute of Health, USA	http://imagej.nih.gov/ij
X-Tile (version 3.6.1)	Camp et al., 2004	http://tissuearray.org
Leica Application Suite LAS X Imaging Software	Leica Microsystems	www.leica-microsystems.com/LAS-X
Gene Cluster 3.0	(Yeung et al., 2001)	Stanford University
Java TreeView (version 1.16r4)	(Yeung et al., 2001)	http://jtreeview.sourceforge.ne