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. 2022 Mar 2;18:9. doi: 10.1186/s13005-022-00313-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Neurosphere development in neuronal medium A. A Neurosphere formation was observed from the third day; appearance of a structure with a condensed center, a perimeter halo, and development in suspension (non-adherent). B On day 5, an increase in the number of neurospheres was observed. C From the seventh day, the beginning an increase in the size of the neurospheres was observed. D Cells in continuous division increasing the dimensions of the spheroid structure in suspension