Table 2.

Marker polymorphism of 20 microsatellites used in this study based on reference population

Locus	k	N	HObs	HExp	HW P-value	Conc. in primer mix (pmol/μL)	References
Multiplex 1
Pmv17	19	233	0.906	0.912	0.50	0.5	[63]
UNH890	6	232	0.414	0.436	0.73	1.0	[64]
UNH908	25	235	0.843	0.875	0.36	3.0	[64]
Gm634	15	234	0.799	0.818	0.42	1.0	[65]
Ppun9	21	233	0.674	0.748	0.02	0.5	[66]
Hchi59	17	232	0.845	0.864	0.52	1.0	[67]
UNH216	11	232	0.603	0.584	0.83	4.0	[68]
UME002	7	228	0.61	0.627	0.44	4.0	[69]
Multiplex 2
Pmv3	31	237	0.768	0.775	0.04	1.0	[63]
GM264	17	234	0.85	0.859	0.42	4.0	[65]
Ppun5	23	233	0.695	0.722	0.19	3.0	[66]
TmoM13	25	234	0.829	0.907	0.44	4.0	[70]
TmoM25	4	231	0.732	0.671	0.55	2.0	[70]
Hchi36	4	230	0.539	0.559	0.44	1.0	[67]
UME003	17	232	0.897	0.869	0.56	2.0	[69]
Multiplex 3
TmoM11	7	234	0.667	0.677	0.87	1.5	[70]
UNH2075	19	233	0.773	0.77	0.23	2.5	[71]
NP101	18	232	0.81	0.749	0.02	3.5	[72]
Pzeb4	8	232	0.612	0.61	0.98	2.0	[73]
UNH974	33	219	0.863	0.926	0.60	4.0	[64]

k: Number of alleles, N: Number of individuals genotyped at the particular locus, H_Obs: Observed Heterozygosity (proportion of heterozygotes at this locus), H_Exp: Expected heterozygosity (expected proportion of heterozygotes given allele frequencies), HW: Adherence to Hardy–Weinberg Equilibrium, tested in Cervus using a Bonferroni correction (Bonferroni corrected α = 0.0025)