Table 1.
Bacterial strains and plasmids used in this study
| Strains or plasmids | Description | Source |
|---|---|---|
| Plasmids | ||
| pMC-Express | A broad host-range shuttle vector derived from pMIDG100, sodC promoter, Cmr | [26] |
| pCZb5 | pMC-Express derivative with a 250 bp fragment containing the tpiA promoter | This work |
| pRE112 | sacB mobRP4 R6K ori Cmr | [24] |
| pCZ52 | pRE112-ΔgatA* | This work |
| pCZ57 | Insertion of complete gatA into pCZb5 | This work |
| pCZ58 | Insertion of complete gatA and hptE into pCZb5 | This work |
| Strains | ||
| SM10 λ pir | E. coli thi thr-1 leu6 proA2 his-4 arg E2 lacY1 galK2, ara14xyl5 supE44, λpir | [25] |
| PM0818 | P. multocida 0818, Wild-type and virulent, LPS genotype L1 | [23] |
| PMZ2 | PM0818 ΔgatA::kanR | This work |