Table 1.
Composition of bovine enteroid monolayer (BEM) media.
| Reagent | Concentration | Supplier |
|---|---|---|
| Advanced DMEM/F12 | 1× | ThermoFisher Scientific (TFS) |
| l-glutamine | 2 mM | TFS |
| HEPES | 10 mM | TFS |
| Penicillin/Streptomycin | 50 U/mL | TFS |
| B27 supplement | 1× | TFS |
| N2 supplement | 1× | TFS |
| EGF (human) | 50 ng/mL | TFS |
| LDN 193189 | 100 nM | Cambridge Bioscience |
| R-Spondin 1 (human) | 100 ng/mL | R&D Systems |
| Noggin (human) | 50 ng/mL | R&D Systems |
| CHIR99021 | 10 µM | Stratech Scientific |
| Y27632 | 10 µM | Cambridge BioScience |
| SB202190 | 10 µM | Enzo Life Sciences |
| LY2157299 | 500 nM | Cambridge BioScience |
| Nicotinamide | 10 mM | Sigma-Aldrich |
| FBS | 1% | GIBCO |