Fig. 4.

Efficiency of IM@ZP under NIR laser irradiation in killing breast cancer cells in vitro. A Calcein AM/PI staining images of 4T1 cells incubated with PBS, IM@Z and IM@ZP with or without NIR laser irradiation (10 min, 1 W/cm²) for 12 h (green fluorescence indicates living cells, and red fluorescence indicates dead cells). B Viability of 4T1 cells incubated with PBS, IM@Z and IM@ZP with or without NIR laser irradiation (10 min, 1 W/cm²) for 12 h measured by CCK-8 assay. *p  < 0.05. C Flow cytometric analysis of cell apoptosis following incubation with PBS, IM@Z and IM@ZP with or without NIR laser irradiation (10 min, 1 W/cm²) for 12 h. *p  < 0.05