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. Author manuscript; available in PMC: 2022 Nov 15.
Published in final edited form as: J Vis Exp. 2021 Nov 10;(177):10.3791/63332. doi: 10.3791/63332

Table of Materials

Name of Material/Equipment Company Catalog Number Comments/Description
18:1 Liss Rhod PE lipid in chloroform Avanti Polar Lipids 810150C
Actin from rabbit skeletal muscle Cytoskeleton AKL99-A
ATTO 488-actin from rabbit skeletal muscle Hypermol 8153-01
Axygen microtubes (200 μL) Fisher Scientific 14-222-262 for handling ABPs
Black resin Formlabs RS-F2-GPBK-04
Cholesterol (powder) Avanti Polar Lipids 700100P
Choloroform Sigma Aldrich 67-66-3
Clear resin Formlabs RS-F2-GPCL-04
Density gradient medium (Optiprep) Sigma-Aldrich D1556
DOPC lipid in chloroform Avanti Polar Lipids 850375C
Fascin homemade N/A
F-buffer homemade N/A
Fisherbrand microtubes (1.5 mL) Fisher Scientific 05-408-129
FS02 Sonicator Fischer Scientific FS20
G-buffer homemade N/A
Glucose Sigma-Aldrich 158968
Mineral oil Acros Organics 8042-47-5
Silicone oil Sigma-Aldrich 317667
96 Well Optical Btm Pit PolymerBase ThermoFisher Scientific 165305
Olympus IX81 Inverted Microscope Olympus IX21
CSU-X1 Confocal Scanner Unit YOKOGAWA CSU-X1
iXon X3 camera Andor DU-897E-CS0
Olympus PlanApo N 60x Oil Microscope Objective Olumpus 1-U2B933