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Representative immunofluorescence staining of cardiac markers and patch-clamping experiment results for iPSC-CMs in the comparison of protocol A and B. (A) Staining of cardiac α-actinin (cACT) on dissociated and (B) cardiac troponin I (cTNNI) and Nkx2.5 on non-dissociated iPSC-CMs. Nuclei were visualized using DAPI. A positive (commercial iPSC-CMs) and negative (iPSC) control staining results are shown for both tested antibody combinations.
