Fig. 4. Isothermal titration calorimetry measurements (ITC) of PPAR ligands and synergy with the coregulator MED1. (A) Comparison of intrinsic affinities (KIID) of the various PPARy ligands as measured by 1D-ITC and 2D-FA (** = not determined). (B) Thermodynamic characteristics of binary interaction of ligand for PPARγ (* = not determined; see additional ITC data & ESI Table S1† for total overview). (C) 2D-ITC of rosiglitazone to PPARγ in the presence of various concentrations of MED1 added in cell and syringe. (D) 2D-ITC of tesaglitazar to PPARγ in the presence of various concentrations of MED1 added in cell and syringe.
