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. 2022 Mar 1;11(1):730–740. doi: 10.1080/22221751.2022.2043727

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Peptide 2 is identified as a CD8+ CTL epitope. Balb/c mice were immunized with the pGX9501. (A) Splenocytes were obtained and used to analyze antigen-specific T-cell induction in the IFN-γ ELIspot assay using in vitro stimulation with the indicated peptides (the peptide with an MHC-I binding RANK < 2 and showing the highest TAP total score or Immunogenicity score in different alleles) was selected for the IFN-γ ELIspot assay. (B) CD4+ T cells and (C) CD8+ T cells were sorted, and specific T-cell induction of IFN-γ-secretion was assayed.