Table 1.

Overlapping Peptide Pool 1.

Peptide number	Sequence	Start	End	aa
1	TAGAAAYYVGYLQPR	258	272	15
2	YYVGYLQPRTFLLKY	264	278	15
3	QPRTFLLKYNENGTI	270	284	15
4	LKYNENGTITDAVDC	276	290	15
5	GTITDAVDCALDPLS	282	296	15
6	VDCALDPLSETKCTL	288	302	15
7	PLSETKCTLKSFTVE	294	308	15
8	CTLKSFTVEKGIYQT	300	314	15
9	TVEKGIYQTSNFRVQ	306	320	15
10	YQTSNFRVQPTESIV	312	326	15
11	RVQPTESIVRFPNIT	318	332	15
12	SIVRFPNITNLCPFG	324	338	15
13	NITNLCPFGEVFNAT	330	344	15
14	PFGEVFNATRFASVY	336	350	15
15	NATRFASVYAWNRKR	342	356	15
16	SVYAWNRKRISNCVA	348	362	15
17	RKRISNCVADYSVLY	354	368	15
18	CVADYSVLYNSASFS	360	374	15
19	VLYNSASFSTFKCYG	366	380	15
20	SFSTFKCYGVSPTKL	372	386	15
21	CYGVSPTKLNDLCFT	378	392	15
22	TKLNDLCFTNVYADS	384	398	15
23	CFTNVYADSFVIRGD	390	404	15
24	ADSFVIRGDEVRQIA	396	410	15
25	RGDEVRQIAPGQTGK	402	416	15
26	QIAPGQTGKIADYNY	408	422	15
27	TGKIADYNYKLPDDF	414	428	15
28	YNYKLPDDFTGCVIA	420	434	15
29	DDFTGCVIAWNSNNL	426	440	15
30	VIAWNSNNLDSKVGG	432	446	15
31	NNLDSKVGGNYNYLY	438	452	15
32	VGGNYNYLYRLFRKS	444	458	15
33	YLYRLFRKSNLKPFE	450	464	15
34	RKSNLKPFERDISTE	456	470	15
35	PFERDISTEIYQAGS	462	476	15
36	STEIYQAGSTPCNGV	468	482	15
37	AGSTPCNGVEGFNCY	474	488	15
38	NGVEGFNCYFPLQSY	480	494	15
39	NCYFPLQSYGFQPTN	486	500	15
40	QSYGFQPTNGVGYQP	492	506	15
41	PTNGVGYQPYRVVVL	498	512	15

Notes:

1. The peptides covered the entire sequence of 258–512 amino acids in the spike protein as previously named as the Pool 2 [15];

2. The peptides were synthesized with an average length of 15 amino acids and nine amino acids overlapping each other.