Table 2.

MHC-I epitope analysis for Overlapping Peptide Pool 1.

Allele	peptide number	MHC-I binding	immunogenicity	Proteasome Score	TAP Score	MHC Score	Processing Score	TAP Total Score
H-2-Dd	21	0.01	0.03612	1.44	1.12	−3.49	2.57	−0.92
H-2-Dd	41	0.04	0.13706	1.74	0.4	−2.56	2.14	−0.42
H-2-Dd	2	0.05	0.1573	1.36	1.07	−3.26	2.43	−0.83
H-2-Dd	26	0.13	−0.02676	1.38	1.27	−4.51	2.65	−1.86
H-2-Dd	9	0.19	−0.11058	1.27	0.99	−3.48	2.26	−1.22
H-2-Dd	19	0.25	0.03263	1.33	1.04	−3.45	2.37	−1.08
H-2-Dd	12	0.29	0.1431	1.05	1.29	−3.9	2.35	−1.55
H-2-Dd	11	0.49	0.1386	1.36	1.21	−4.02	2.57	−1.45
H-2-Dd	32	0.62	0.0966	1.19	1.17	−3.83	2.36	−1.46
H-2-Dd	37	0.65	0.12191	1.38	1.09	−3.76	2.47	−1.29
H-2-Dd	14	0.67	0.08562	1.39	0.82	−3.76	2.21	−1.55
H-2-Dd	31	0.7	0.023	1.31	1.15	−4.17	2.46	−1.71
H-2-Dd	20	0.7	−0.31841	1.47	1.3	−4.6	2.77	−1.83
H-2-Dd	27	0.89	−0.11289	1.4	1.21	−4.54	2.61	−1.92
H-2-Dd	28	1.1	−0.19576	0.98	1.12	−4.22	2.1	−2.12
H-2-Dd	24	1.2	0.12947	1.27	0.71	−4.63	1.98	−2.64
H-2-Dd	25	1.2	−0.11559	1.43	0.15	−4.39	1.58	−2.81
H-2-Dd	18	1.2	−0.22309	1.33	1.17	−4.05	2.5	−1.55
H-2-Dd	40	1.3	0.1256	1.38	1.34	−4.5	2.71	−1.79
H-2-Dd	1	1.3	0.06158	1.24	1.23	−4.32	2.47	−1.85
H-2-Dd	33	1.5	−0.21085	1.03	1.24	−4.23	2.27	−1.96
H-2-Dd	29	1.6	0.05792	1.52	0.5	−4.17	2.02	−2.15
H-2-Dd	30	1.6	0.05792	1.35	0.46	−4.17	1.8	−2.36
H-2-Dd	39	1.6	−0.15021	1.25	1.15	−3.84	2.41	−1.44
H-2-Dd	10	2	0.01977	1.1	0.24	−4.23	1.34	−2.89
H-2-Kd	2	0.01	0.06572	1.24	0.48	−1.78	1.72	−0.06
H-2-Kd	41	0.05	0.04196	1.74	0.46	−3.43	2.2	−1.22
H-2-Kd	9	0.06	0.12441	1	0.23	−2.55	1.23	−1.32
H-2-Kd	4	0.07	0.28634	1.34	0.37	−2.34	1.71	−0.63
H-2-Kd	3	0.07	0.05892	1.16	0.37	−2.34	1.54	−0.8
H-2-Kd	20	0.08	0.25644	1.45	0.49	−2.65	1.93	−0.71
H-2-Kd	21	0.08	0.05832	1.75	0.36	−2.65	2.12	−0.53
H-2-Kd	16	0.11	0.16858	1.36	0.4	−2.32	1.77	−0.55
H-2-Kd	40	0.19	0.0905	1.38	1.32	−3.9	2.69	−1.21
H-2-Kd	23	0.26	0.0573	1.31	0.44	−3.18	1.75	−1.43
H-2-Kd	24	0.61	−0.0378	1.07	0.2	−3.02	1.27	−1.75
H-2-Kd	19	0.68	0.0279	1.33	1.2	−3.27	2.52	−0.75
H-2-Kd	10	0.79	0.034	1.1	0.23	−3.35	1.33	−2.02
H-2-Kd	12	0.92	0.34063	1.45	0.59	−3.13	2.03	−1.1
H-2-Kd	1	0.96	−0.04018	1.24	1.29	−4.51	2.53	−1.98
H-2-Kd	32	0.99	0.13255	1.19	1.18	−3.56	2.37	−1.19
H-2-Kd	39	1.1	0.0801	1.25	1.31	−3.16	2.56	−0.6
H-2-Kd	31	1.2	0.1811	1.48	0.48	−3.32	1.96	−1.36
H-2-Kd	29	2	0.07062	1.52	0.5	−3.5	2.02	−1.48
H-2-Kd	30	2	0.07062	1.35	0.46	−3.5	1.8	−1.7
H-2-Kd	35	2	−0.15381	1.42	1.16	−4.52	2.57	−1.95
H-2-Ld	11	0.06	0.30371	1.36	0.98	−3.5	2.34	−1.16
H-2-Ld	41	0.12	−0.07228	1.74	0.35	−3.54	2.09	−1.45
H-2-Ld	21	0.2	0.05832	1.44	0.99	−3.65	2.44	−1.21
H-2-Ld	39	0.27	−0.19696	1.25	0.91	−3	2.16	−0.84
H-2-Ld	12	0.28	0.09851	1.05	0.94	−2.75	2	−0.75
H-2-Ld	37	0.44	0.0801	1.38	0.99	−4.01	2.37	−1.65
H-2-Ld	2	0.54	0.28634	1.41	1.19	−3.97	2.6	−1.38
H-2-Ld	3	0.57	−0.08994	1.52	1.13	−3.97	2.65	−1.32
H-2-Ld	27	0.77	0.0573	1.4	1.21	−4.39	2.61	−1.78
H-2-Ld	28	0.77	0.03448	0.98	1.12	−4.18	2.1	−2.07
H-2-Ld	19	0.95	0.11915	1.53	1.42	−4.32	2.95	−1.37
H-2-Ld	18	1.1	−0.22669	1.33	1.17	−3.82	2.5	−1.32
H-2-Ld	34	1.9	0.1811	0.91	1.16	−4.58	2.07	−2.5
H-2-Ld	9	1.9	−0.08994	1.27	0.99	−4.13	2.26	−1.87
H-2-Ld	38	2	−0.19696	0.99	0.28	−2.8	1.27	−1.53

Notes:

1. MHC-I binding score was between 0 and 2. < 0.5 strong binder, 0.5-2 weak binder, > 2 non-binder.

2. A high Immunogenicity score indicates that the degree of the peptide conformity to sequence preference was good.

3. The higher the TAP total score, the higher the likelihood that the peptide will be presented after being swallowed by DCs.