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. 2022 Feb 28;44(1):368–380. doi: 10.1080/0886022X.2022.2039195

Figure 7.

Figure 7.

MiR-142-5p targets SIRT7 during cisplatin treatment in HK-2 cells. (A) Conserved putative miR-142-5p targeting site in the 3′ UTR of SIRT7 mRNA, as identified using online databases (TargetScan); (B) immunoblotting analysis of SIRT7 protein expression levels; (C) quantitative analysis of the expression of SIRT7 (*p < 0.05 versus scramble, #p < 0.05 versus cisplatin); (D) double luciferase assay carried out to confirm the target gene of miR-142-5p after co-transfection of SIRT7 3′ UTR double luciferase reporter gene vector and miR-142-5p mimics; (E) SIRT7 level examined by Western blot after miR-142-5p treatment in mice induced by cisplatin; F. quantification of SIRT7 by densitometry. Data were expressed as mean ± SD (n = 3); *p < 0.05 versus control, #p < 0.05 versus cisplatin.