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. 2022 Feb 27;14(1):2041790. doi: 10.1080/20002297.2022.2041790

Figure 2.

Figure 2.

NSA inhibited the expression of necroptosis associated genes as well as TLR2. HIOECs were stimulated with P. gingivalis LPS at 1 µg/mL for 24 h. NSA at 2 or 4 µM was applied for 2 h before LPS stimulation. a, b. Western blots of TLR2 and necroptosis-associated proteins and bar graphs of relative fold changes of Western blots. c, d. NSA at 4 µM was applied for 2 h before LPS stimulation, then HIOECs were stimulated with P. gingivalis LPS at 1 µg/mL for 48 h. Expression of MLKL in red fluorescence and bar graphs of relative fold changes of MLKL. Scale bars, 50 µm. *, Significant difference compared to the control group. #, Significant difference compared to the LPS group. *, P < 0.05. #, P < 0.05. ##, P < 0.01.