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. 2022 Feb 27;14(1):2041790. doi: 10.1080/20002297.2022.2041790

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — NSA blocked cell death, destruction of epithelial connection as well as DAMPs expression. HIOECs were stimulated with P. gingivalis LPS at 1 µg/mL for 24 h. NSA at 4 µM was applied for 2 h before LPS stimulation. a, b. Data of cell viability and LDH release with or without NSA. c, d. Effect of necroptosis on oral epithelial connection by E-cadherin immunofluorescence staining and bar graph. Red fluorescence indicates E-cadherin protein. Scale bars, 100 µm. e. Gene expression profiles of DAMPs by qPCR. *, Significant difference compared to the control group. #, Significant difference compared to the LPS group. *, P < 0.05. **, P < 0.01. ***, P < 0.001. ****, P < 0.0001.#, P < 0.05. ##, P < 0.01. ###, P < 0.001.