Figure 5.

Analysis of lymphocytes in the mesenteric lymph nodes (mLN) and colon. (A) Immunohistochemical staining for CD3⁺ and CD4⁺ T cells and FoxP3⁺ Tregs in mLN from normal (Con) and experimental colitis mice treated with saline (Mod), DCs, or DCTP. Significant decrease in CD3⁺ and CD4⁺ T cells and increase in FoxP3⁺ Tregs were found in DCTP-treated mice compared with mice in the DC and mod groups. (B) FCM analysis of the CD3⁺, CD4⁺, and FOXP3⁺ T cell percentages in mLN showed an increase in Tregs and decrease in CD3⁺ and CD4⁺ T cells in the DCTP group. Experiments were repeated three times in quintuplicate each time (n = 15). (C) Immunohistochemical staining for CD3⁺ and CD4⁺ T cells and FoxP3⁺ Tregs in the colon of normal (Con) and experimental colitis mice treated with saline (Mod), DCs, or DCTP. Significant decreases in CD3⁺ and CD4⁺ T cells and an increase in FoxP3⁺ Treg infiltration were found in the colon of mice in the DCTP group compared with that of mice in the DC and Mod groups. Equivalent larger versions were shown in upper right corner for better observation (the scale bar represents 100 µm; two-tailed t-test, *p<.05, **p<.01).