TABLE 6.

Summary of the M27 method developed by the NCCLS^a

Factor	Implementation in the M27 methodology
Methodology	Broth macrodilution, 1-ml final volume; or broth microdilution, 0.2-ml final volume
Medium	RPMI 1640 containing 0.165 M MOPSb (pH 7.0)
Fungal inoculum	(0.5–2.5) × 103 organisms
Incubation temp	35°C
Incubation time	48 h (Candida species) or 72 h (Cryptococcus neoformans)
End point	Amphotericin B, optically clear tube; azoles and 5-FC, 80% reduction in turbidity by comparison with growth control
Drug and quality control (QC) isolates	Two QC isolates and corresponding QC ranges established via the M23 procedure are specified for amphotericin B, flucytosine, ketoconazole, itraconazole, and fluconazole

^aAdapted from reference 217 with permission of the publisher. 

^bMorpholinepropanesulfonic acid.