TABLE 2.

Oligonucleotides used in binding assays. SμG oligo can adopt a G4 conformation while the M1 oligos cannot. Guanine-runs in SμG oligo are in bold. G>A mutations in M1 oligo introduced to disrupt guanine-runs are indicated as lowercase letters.

Oligo	Sequence	Length	G-score*
SμG	5′GAGCTGGGGTGAGCTGGGCTGAGCTGGGGTGAGCTGGGCTGAGCT	45 nt	70
M1	5′GAGCTGaGGTGAGCTGGGCTGAGCTGaGGTGAGCTGGGCTGAGCT	45 nt	N/A

^*G-score was calculated using QGRS Mapper (https://bioinformatics.ramapo.edu/QGRS/index.php; parameters: Max Length – 44, Min G-Group Size – 3, and Loop Size – 0 to 10).