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. 2022 Feb 17;16(2):e0010186. doi: 10.1371/journal.pntd.0010186

Fig 4. Application of the competitive ELISA assay for the detection of WNV anti-pr-E antibodies in 10 chicken sera from Crete (samples S1-S10, 2018).

Fig 4

PC: positive control, NC: negative control. Absorbance (Abs) values in 450 nm are given in the parentheses. The % Signal to Noise (%S/N) is calculated as: %S/N = (Abs_sample/Abs_negative) x 100%. The result is considered positive if %S/N ≤ 40.0%, doubtful if %S/N falls between 40.0%-50.0% and negative if %S/N > 50.0% Sample S5 is positive (%S/N = 5.0%), whereas the remaining samples are negative.