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. Author manuscript; available in PMC: 2022 Mar 2.
Published in final edited form as: J Control Release. 2020 May 5;324:172–180. doi: 10.1016/j.jconrel.2020.04.054

Figure 1. FUS setup and targeting procedure.

Figure 1.

(a) FUS benchtop setup. (b, from left to right)) Animals are first positioned in a 3-D printed stereotaxic frame equipped with an MRI fiducial (red arrow, left image). The frame is then placed inside the MRI bed and distance from the fiducial (dotted circle) to the target brain region is measured using both coronal images for the dorsal/ventral measurements and axial images for the rostral/caudal measurements, medial/lateral measurement can be collected from both axes. Animals are kept in the frame and transferred to the FUS station where a pointer is used to zero the XYZ positioner at the location of the fiducial. The pointer is then replaced with the FUS transducer (FUS Instruments) and a water bath is coupled to the animal’s head with gel. Lastly, using the XYZ positioner and the measurements collected from the MR images, the transducer focal point is positioned over the target brain site.