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. 2022 Feb 23;2022:5503575. doi: 10.1155/2022/5503575

Figure 3.

Figure 3

Knockdown of CBX4 increased senescence markers in WI-38 cells. Knockdown (KD) of CBX4 was performed with CBX4 shRNA carried by lentiviral particles with different multiplicity of infection (MOI) 0.5, 1, and 2. (a) Representative Western blot of CBX4 protein expression in proliferating (positive control CPD30) and senescent (negative control CPD60) WI-38, in CBX4 KD and lentiviral control (Ctrl) at three MOI. (b) Overall significant reduction of CBX4 protein (n = 14) and gene expression (n = 11) due to lentiviral KD (control grey, CBX4 KD light blue). (c) and (d) SA-β-gal activity, p16 and EdU proliferation expression in control and CBX4 KD in representative flow cytometry (C), and overall demonstrating increased protein expression of senescence markers SA-β-gal activity and p16, and decreased proliferation marker EdU after knockdown of CBX4 ((d), n = 11).