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. 2022 Feb 23;2022:5503575. doi: 10.1155/2022/5503575

Figure 5.

Figure 5

Regulation of senescence-related genes by CBX4. A selected panel of senescence-related genes was analyzed by qPCR microarray. Two serial culture experiments were performed, and the resulting Log2FC values were computed (normalized with YWHAZ and calculated relative to the earliest CPD of serial culture). (a) and (b) Heatmaps of standardized gene expression at 10 time points of WI-38 serial culture performed in two biological replicates. (c) the Rmcorr correlation (and 95% confidence intervals) of CPD with expression (Log2 FC) of 37 senescence-related genes from serial culture. Green: positive correlation of gene expression with CPD. Red: negative correlation gene expression with CPD. Black: insignificant correlation of gene expression with CPD. (d) Heatmap of pairwise Spearman correlations of genes measured in the serial culture experiment. Based on expression pattern and consistent with (a) and (b), two groups of genes were highly clustered. (e) Volcano plot of gene expression changes in response to CBX4 KD vs. Ctrl showing increased expression of DPP4, CDKN2A, and SLC52A1 and decreased expression of CXCL8, PCNA, DNMT1, E2F1, and PARP1. (f) Volcano plot of gene expression changes in response to CBX4 ACT vs. Ctrl showing decreased expression of DPP4, CDKN2A, and HDAC1 and increased expression of CBX4, SIRT1, and MDM4. Red: increased expression, green: decreased expression.