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. 2022 Feb 23;603(7899):119–123. doi: 10.1038/s41586-022-04428-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — Holding currents (solid lines, top), membrane potentials (solid lines, centre), and input resistances (dashed lines, bottom) of exemplary T4 neurons expressing either GFP (a) or GFP + GluClα^RNAi (b). To obtain input resistance measurements at high temporal resolution, neurons were subjected to at least two repetitions of identical visual stimulation while recording their membrane potentials. In this case, the stimulus was an ON edge moving at 30° s⁻¹ in the neuron’s preferred direction. The holding current I was altered in between the first (#1) and the second repetition (#2) by ΔI = −1 pA. The input resistance R_in at each time point was calculated as ΔV_m/ΔI, where ΔV_m denotes the difference in membrane potential between repetitions (shaded areas/dashed arrows in a).

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