Extended Data Fig. 9. Binding of TDP-43 to SNP-containing intronic RNA.

(a–d) ITC measurement of the interaction of TDP-43 with 14-nt RNA containing the CE SNP (a, b) and intronic SNP (c, d) healthy sequence. A representative data set is reported, with raw data (a, c) and integrated heat plot (b, d). Circles indicate the integrated heat; the curve represents the best fit. (e) Raw Tapestation gel images corresponding to Fig. 4j. For each experiment, two RT-PCRs were performed with a different primer set which either amplified a control minigene (top row; minigene 2H) or a mutant minigene (bottom row). Left: single transfections to ensure specificity of primers for either the control or the mutant minigene. Right: Three replicates of double transfections with control minigene 2H and either mutant minigene.