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. Author manuscript; available in PMC: 2022 Mar 3.
Published in final edited form as: Nat Chem Biol. 2021 Dec 16;18(3):313–320. doi: 10.1038/s41589-021-00914-3

Fig. 2. E-Syt C2 domains reversibly and sequentially bind to membranes.

Fig. 2.

(a, c, d) Extension-time trajectories at constant mean force F for the C2 repeat E-Syt1 C2ABCDE (a), E-Syt1 C2E (c), or E-Syt2 C2ABC (d). The average extensions of three states (numbered on the left as in Fig. 1d) are marked by green dashed lines. A close-up view of the indicated region in the second trajectory in a is shown in the fourth trajectory. The overlaying red trace represents an idealized state transition derived from hidden-Markov modeling (HMM), as in other extension-time trajectories. (b, e) Probability density distributions of the extensions shown in a or d (symbols) and their best fits with a sum of three Gaussian functions (solid curves), with the individual Gaussian functions shown as dashed curves for the black curves.