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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Free Radic Biol Med. 2022 Feb 5;181:143–153. doi: 10.1016/j.freeradbiomed.2022.01.032

Figure 6. TIGAR is down-regulated by oxidative stress and upregulated by antioxidants and DCA.

Figure 6.

(A) Immunoblot of retinal homogenates of rd10 mice at P35 given no treatment (Control, n=6) or treated with 13 mM DCA (n=6) immunoblotted with anti-TIGAR antibody and anti-actin antibody. (B) Densitometry of blot in (A) showed a significant increase in TIGAR/Actin ratio in retinas of mice treated with DCA (p=0.0130). (C) Wild type C57BL/6 mice were given an intraocular injection of 1.5 mM paraquat (n=7) or no treatment (Control, n=7) and after 16 hours they were euthanized and retinal homogenates were immunoblotted with anti-TIGAR antibody and anti-Actin antibody. (D) Densitometry of the blot in (C) showed a significant decrease in TIGAR/actin ratio in retinas from eyes injected with 1.5 mM paraquat. (E) Retinal homogenates from C57BL/6 mice (n=7) and rd10 mice (n=7) at P35 were immunoblotted with anti-TIGAR antibody and anti-Actin antibody. (F) Densitometry of blot in (E) showed a significant decrease in TIGAR/Actin ratio in retinas of rd10 mice. (G) Starting at P8, rd10 mice were given drinking water supplemented with 7 mg/ml N-acetylcysteine (NAC, n=7) or unsupplemented water (Control, n=7) and at P50 mice were euthanized and retinal homogenates were immunoblotted with anti-TIGAR antibody and anti-Actin antibody. (H) Densitometry of the blot in (G) showed a significant increase in TIGAR/actin ratio in retinas of mice treated with NAC (p=0.0379). (I) Starting at P8, rd10 mice were given drinking water supplemented with 42 mM glucose and 10 mM pyruvate (n=7) or unsupplemented water (Control, n=7) and at P50 mice were euthanized and retinal homogenates were immunoblotted with anti-TIGAR antibody and anti-Actin antibody. (J) Densitometry of the blot in (I) showed an increase in TIGAR/Actin ratio in retinas of mice treated with 42 mM glucose and 10 mM pyruvate in drinking water.

* p ≤ 0.05, *** p ≤ 0.001 by Mann-Whitney test.