Fig. 2.

Favourite similars buf-s-14, buf-s-18 and lyco-s have low toxicity and inhibit HCF migration. a In addition to efficacy (BrdU, teal; WST-1, green), we determined cytotoxicity via dye inclusion (CellTox Green, not shown), LDH release (red) as well as Caspase-3/7 activation (yellow). Vertical lines represent EC50, horizontal lines 95% CI. Horizontal bars indicate therapeutic index (T.i., IC50 ∕ EC50) until LDH (red) or caspase activation (yellow). Pale part of bars indicates predicted range above the highest tested concentration. n = 3 biological replicates (donors), 2 technical replicates each. b Effective concentration (EC50 by BrdU assay) and T.i. (IC50 / EC50) for working set. CTG: CellTox Green dye. c, d HCF were stained with 1:1000 DIR before plating. After 24 h incubation, cells were scratched and subsequently treated with the compounds (c). All compounds were able to inhibit HCF migration (d). n = 3 biological replicates, six technical replicates each. Bars represent mean ± 95% CI. Analysed with Two-way ANOVA (p < 0.05), adjusted following Dunnett. DIR: 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindotricarbocyanine Iodide