Table 2.

Validation parameters of the method for the studied analytes in rat plasma.

Parameter	Hydroxychloroquine	Favipiravir	Oseltamivir	Remdesivir
Linear range (ng/mL)	5–100	50–1000	5–100	10–100
Mean ± Standard deviation (S.D)	99.75 ± 0.75	98.01 ± 1.41	99.63 ± 1.31	100.04 ± 1.67
R.S.D*	0.75	0.14	1.31	1.67
Regression Equation	Y = 0.0277 X -0.0079	Y = 0.0003X + 0.021	Y = 0.0235X + 0.1993	Y = 0.0197X ± 0.1648
Correlation Coefficient (r)	0.9999	0.9998	0.9999	0.9999
Intraday precision (%R.S.D)	0.83	0.47	2.30	1.10
Interday Precision (%R.S.D)	0.60	0.85	0.52	0.55
Limit of Detection (L.O.D)** (ng/mL)	0.11	10.00	0.92	1.71
Limit of Quantitation (L.O.Q)*** (ng/mL)	0.36	33.33	3.07	5.72

LOD and LOQ are determined based on signal to noise ratio.

RSD*: relative standard deviation before

LOD** =3 × S/N.

LOQ***=10 × S/N.