Table 3.

Absolute Recovery and matrix effect at five different concentration levels for the studied analytes in rat plasma using UHPLC-MS/MS (n = 15).

Name of Analyte	Absolute Recovery		Matrix effect
	Spiked level (ng/mL)	Found (ng/mL)	Rec % *	Spiked level (ng/mL)	Found (ng/mL)	Matrix Effect (ME %)*
Entecavir (IS)	50	52.94	105.88	50	43.10	86.20
	50	53.89	107.78	50	48.99	97.98
	50	50.82	101.64	50	46.58	93.15
	50	55.55	111.1	50	45.75	91.50
	50	51.48	102.96	50	44.38	88.75
			Rec ± S.D	105.87 ± 3.79	Rec ± S.D	91.52 ± 4.48
Hydroxychloroquine	5	3.92	78.4	5	4.25	85.00
	10	8.00	80	10	8.49	84.90
	30	32.46	108.2	30	24.22	80.73
	70	76.33	109.04	70	58.18	83.11
	100	105.82	105.82	100	87.73	87.73
			Rec ± S.D	96.31 ± 15.64	Rec ± S.D	84.29 ± 2.59
Favipiravir	50	50.11	100.22	50	54.23	108.46
	100	91.93	91.93	100	112.52	112.52
	300	270.84	90.28	300	320.28	106.76
	500	558.65	111.73	500	578.2	115.64
	1000	851.30	85.13	1000	1195	119.5
			Rec ± S.D	95.86 ± 10.40	Rec ± S.D	112.58 ± 5.20
Oseltamivir	5	5.55	111	5	5.58	111.65
	20	17.89	89.45	25	28.66	114.64
	50	45.81	91.62	50	53.1	106.20
	90	74.53	82.81	75	87.31	116.41
	100	83.92	83.92	100	113.08	113.08
			Rec ± S.D	91.76 ± 11.37	Rec ± S.D	112.40 ± 3.89
Remdesivir	10	7.81	78.1	10	10.06	100.60
	30	25.04	83.47	30	31.04	103.47
	50	42.77	85.54	50	51.64	103.28
	70	56.34	80.49	70	78.38	111.97
	100	84.37	84.37	100	102.48	102.48
			Rec ± S.D	82.39 ± 3.04	Rec ± S.D	104.36 ± 4.40

Average of 5 determinations*