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. 2022 Feb 17;10:805402. doi: 10.3389/fcell.2022.805402

FIGURE 2.

FIGURE 2

Correlation between INMT and tumor signaling pathways. (A,B) In TCGA and GSE46602, INMT was enriched in prostate cancer, MAPK signaling, TGFβ signaling, and Wnt signaling pathways. After transfection with corresponding negative control lentiviral vector and INMT overexpression lentiviral vector in 22Rv1 and PC-3, (C) mRNA levels of INMT were measured using real-time PCR, and (D,E) protein levels of INMT were measured using western blot. (D,F) Protein levels of MAPK signaling pathway-related molecules (Phospho-p38 MAPK, p38 MAPK) were measured using western blotting. (D,G) Protein levels of TGFβ signaling pathway-related molecules (TGFβ1, Smad2, and Smad4) were measured using western blot. (D and H) The protein levels of Wnt/β-catenin signaling pathway-related molecules (β-catenin and cyclin D1) were measured using western blot. The samples derive from the same experiment, and those blots were processed in parallel.