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. 2022 Feb;192(2):239–253. doi: 10.1016/j.ajpath.2021.10.020

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© 2022 American Society for Investigative Pathology. Published by Elsevier Inc.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Preservation of ciliary beat in human precision-cut lung slices (PCLS) during long-term cultivation. The PCLS derived from immediately processed or overnight stored fibrotic explants as well as tumor lung resections. Ciliary beat was assessed on each day of medium change, and the relative number of PCLS with ciliary beat was calculated compared with the initial number of PCLS with ciliary beat. Weighted means ± SD were used for the statistical analysis of all data points. Graphs were generated from weighted means of a limited number of data points (week 1 = day 7 ± 1, week 2 = day 14 ± 1, week 3 = day 21 ± 1, week 4 = day 28 ± 1) for illustration purposes. Data are expressed as means ± SD. ∗P < 0.05. n, number of PCLS; N, number of lungs.