Skip to main content
. 2021 Dec 27;152(3):747–757. doi: 10.1093/jn/nxab429

FIGURE 1.

FIGURE 1

Experimental design and group designations for iron repletion and choline supplementation of primary mouse hippocampal neuron culture model of iron deficiency. The general in vitro timeline of key neurodevelopmental processes is shown at the bottom. Numbers indicate the number of days of in vitro culture after harvesting and plating cells from the embryonic day 16 (E16) wild-type, mouse hippocampus. Each of the 6 treatment groups are indicated by a bar between 3 DIV and 18 DIV. Gray bars indicate the time period of DFO treatment and thus culture in iron-deficient media. White bars indicate the days without DFO treatment and thus culture in iron-sufficient media. The vehicle is sterile distilled, deionized water (same volume as DFO). Iron repletion between 11 DIV and 18 DIV was accomplished by removal of DFO and subsequent culture in iron-sufficient media. Black arrows between 11 DIV and 18 DIV indicate the period of choline treatment. The group designations for each of the 6 treatment groups are shown on the right. Cho, choline; DFO, deferoxamine; DIV, days in vitro; FID, formerly iron deficient; ID, iron deficient; IS, iron sufficient.