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. 2022 Mar 2;79(3):168. doi: 10.1007/s00018-022-04205-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — The meprin β knockout has no evident effect on the overall APP processing and its secretases. A Soluble brain fractions of 15-month-old APP/lon and APP/lon × Mep1b^−/− mice (n = 10/group) were analyzed by immunoblot for sAPP forms using the monoclonal antibody 22C11. Further immunoblot analysis of insoluble brain fractions using an antibody against the C-terminus of full-length APP and CTFs in 15-month-old APP/lon and APP/lon × Mep1b^−/− mice (n = 10/group). B Immunoblot analysis shows protein levels for Notch1 and (n = 8/group) its fragments (n = 4/group). C The protein levels for ADAM10 in insoluble brain fractions show no differences between the groups (n = 8/group). D γ-Secretase subunits PSEN1 and Nicastrin in insoluble fractions of APP/lon and APP/lon × Mep1b^−/− mice (n = 5/group). E Total brain lysates from double knockout mice for BACE1 and meprin β, as well as Mep1b^−/− and WT mice were analyzed by immunoblot for Sez6. A–D Densitometric analyses normalized to β-tubulin revealed no significant differences. E Densitometric analysis normalized to GAPDH detected an increase in levels of endogenous Sez6 in the double knockout mice lacking BACE1, but no significant differences were found in meprin β knockout compared to WT brain homogenates