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. 2022 Mar 2;12:3427. doi: 10.1038/s41598-022-07408-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Assay protocol. Each tryptophanyl-tRNA synthetase (TrpRS), glycyl-tRNA synthetase (GlyRS), histidyl-tRNA synthetase (HisRS), and lysyl-tRNA synthetase (LysRS) was dispended to each enzymatic reaction area. Five percent of ammonium molybdate in 2.5 M sulfuric acid was dispensed to the detection area. The analyte solution was loaded onto the sample spot of the LPAD, and the spot area and the enzymatic reaction areas were heated at 60 °C using an aluminum heating block.