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. 2022 Mar 2;13:1127. doi: 10.1038/s41467-022-28753-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a–c The localization of GFP-tagged HAKAI in roots of 5-day-old HAKAIpro:HAKAI-GFP/hakai. a A primary root tip. b A lateral root initiation site. c A formed lateral root. Scale bar = 50 μm. Experiments in a–c were repeated independently at least three times, and representative images are shown. d m⁶A levels checked by two-dimensional thin layer chromatography (TLC) analysis. Data represent mean ± SE from three biological replicates and statistically significant differences relative to WT were analyzed by two-sided unpaired t-test (n.s. no significance). p = 0.7845. e–h Volcano plots showing proteins co-purified with HAKAI or MTA. The distribution of quantified proteins was plotted according to the Log₂ fold change of label free quantification (LFQ) intensities and −Log₁₀^p-value obtained from two-sided student t-test of three independent experiments. Significantly enriched proteins were separated from others by a hyperbolic curve. Proteins indicated with filled squares represent known m⁶A writer complex members (red) and additional components identified in this work (blue). e HAKAIpro:HAKAI-GFP/hakai versus WT. HIZ1 and HIZ2 refer to HAKAI-interacting zinc finger protein 1 and 2 (AT1G32360 and AT5G53440, respectively). MTB is indicated not directly interacting with HAKAI, labeled with a black filled square. f MTApro:MTA-GFP/mta versus WT. g hakai-2 MTApro:MTA-GFP/mta versus WT. h hakai-2 MTApro:MTA-GFP/mta versus MTApro:MTA-GFP/mta. Source data are provided as a Source Data file.