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. 2022 Mar 2;13(3):202. doi: 10.1038/s41419-022-04649-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b qPCR (a) and western blot (b) detecting the mRNA and protein level of INHBA in NCM460 and CRC cells. c, d qPCR (c), and western blot (d) testing the effect of metformin (10 mM, 24 h treatment) on the expression of INHBA. e ELISA measured the secretion of activinA after metformin treatment. f–h qPCR (f), western blot (g), and IHC (h) evaluating the expression change of INHBA in subcutaneous tumors from control and metformin treatment groups. i Representative IHC images of CRC tissues with or without metformin treatment stained with an INHBA antibody (n = 22). Up: Magnification: 50×; Scale bar: 200 µm. Low: Magnification: 200×; Scale bar: 50 µm. The values are shown as the mean ± SD and experiments were independently repeated three times. *P < 0.05, ***P < 0.001. The data in this figure were analyzed by Student’s t test.