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. 2022 Mar 2;13(3):202. doi: 10.1038/s41419-022-04649-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b Western blot (a), and qPCR (b) was used to confirm the efficiency of INHBA overexpression. c The concentration of INHBA in a conditioned medium of 1 × 10⁶ cells was determined by ELISA. d–f EdU (d), CCK8 (e), and flow cytometry (f) showing the promotion effect of INHBA on tumor cell growth. g Western blot analyzing the protein level of INHBA. INHBA-GFP: the exogenous fusion protein. h CCK8 analyzing the effect of metformin on cell viability after overexpression INHBA. i CCK8 detecting the influence of condition medium from the INHBA overexpression cells on metformin inhibiting SW480 cell growth. CM: condition medium from the cells overexpressing INHBA. The values are shown as the mean ± SD and experiments were independently repeated three times. *P < 0.05, ***P < 0.001. The data in this figure were analyzed by two-way ANOVA.