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. 2022 Mar 2;13(3):200. doi: 10.1038/s41419-022-04652-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Real-time PCR analysis of miR-183-5p.1 in spheroids generated from SOX2 knockdown hepatoma cells and control cells. B Real-time PCR analysis of miR-183-5p.1 in spheroids generated from SOX2 overexpression hepatoma cells and control cells. C The correlation between the level of SOX2 and miR-183-5p.1 in primary HCC cells (n = 30) was determined by real-time PCR analysis. D Real-time PCR analysis of pri-miR-183-5p.1 in spheroids generated from SOX2 knockdown hepatoma cells and control cells. E The luciferase reporter activity of miR-183-5p.1 promoter was measured in SOX2 knockdown and control HCC spheres. F Hepatoma cells subjected to ChIP assay with anti-SOX2 or anti-IgG antibody. G The luciferase reporter activity of miR-183-5p.1-WT or miR-183-5p.1-Mut promoter was measured in SOX2 knockdown and control HCC spheres, and the relative activity was presented (relative to control). H The luciferase reporter activity of miR-183-5p.1-WT or miR-183-5p.1-Mut promoter was measured in SOX2 overexpression and control HCC spheres, and the relative activity was presented (relative to control). I Western blot analysis of indicated proteins in spheroids generated from SOX2 overexpression hepatoma cells and control cells. J Western blot analysis of indicated proteins in spheroids generated from SOX2 knockdown hepatoma cells and control cells. All results are presented as the mean ± SD, and statistical significance was assessed using a two-tailed Student t-test. *p < 0.05.