Figure 5.
Pharmacological modulation of axolotl MC3R and MC4R by α-MSH and ACTH. (A) Effect of different doses (10−12–10−6 M) of α-MSH in activating axolotl MC3R in the presence of axolotl MRAP2 in HEK293T cells measured with Dual-glo luciferase assay. (B) Effect of different doses (10−12–10−6 M) of ACTH in activating axolotl MC3R in the presence of axolotl MRAP2 in HEK293T cells measured with Dual-glo luciferase assay. (C) Effect of different doses (10−12–10−6 M) of α-MSH in activating axolotl MC4R in the presence of axolotl MRAP2 in HEK293T cells measured with Dual-glo luciferase assay. (D) Effect of different doses (10−12–10−6 M) of ACTH in activating axolotl MC4R in the presence of axolotl MRAP2 in HEK293T cells measured with Dual-glo luciferase assay. (E, F) The constitutive activity of MC3R/MC4R in the presence of different amounts of MRAP2. ****p < 0.0001.