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. 2022 Feb 17;13:820896. doi: 10.3389/fendo.2022.820896

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Copyright © 2022 Wang, Xue, Lei, Song, Li, Li, Fu, Zhang, Zhang, Luo, Wang, Lin, Zhang and Guo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interaction and co-localization of axolotl MC3R/MC4R and MRAP2 in vitro. (A) Coimmunoprecipitation of 3HA‐MC3R with 2xFlag‐MRAP2 in HEK293T cells. (B) Coimmunoprecipitation of 3HA‐MC4R with 2xFlag‐MRAP2 in HEK293T cells. (C) Confocal microscopy of the co-localization of MRAP2-VF1 and MRAP2-VF2 on the plasma membrane. (D) Confocal microscopy of the co-localization of MC3R and MRAP2 on the plasma membrane. (E) Confocal microscopy of the co-localization of MC4R and MRAP2 on the plasma membrane. Nuclei stained with DAPI are shown in blue and Venus fluorescence in green. Surface expression of MC3R/MC4R, and MRAP2 is shown in red, detected by anti‐Flag antibody and secondary anti‐rabbit Alexa Fluro 594 (Abcam). Scale bar = 50 µm.