Table 1.
EC50 values of α-MSH and ACTH in activating axolotl MC3R/MC4R in presence of different amounts of axolotl MRAP2.
| 1:0 | 1:1 | 1:3 | 1:6 | ||
|---|---|---|---|---|---|
| MC3R:MRAP2 (α-MSH) | logEC50 | −9.95 ± 0.35 | −9.29 ± 0.21 | −9.04 ± 0.13 | −8.98 ± 0.13 |
| P-value | ns | *** | **** | ||
| MC3R:MRAP2 (ACTH) | logEC50 | −9.51 ± 0.36 | −9.47 ± 0.51 | −9.40 ± 0.28 | −9.55 ± 0.14 |
| P-value | ns | ** | *** | ||
| MC4R:MRAP2 (α-MSH) | logEC50 | −9.84 ± 0.31 | −10.02 ± 0.40 | −9.37 ± 0.22 | −9.06 ± 0.22 |
| P-value | ns | ns | ns | ||
| MC4R:MRAP2 (ACTH) | logEC50 | −9.62 ± 0.30 | −10.13 ± 0.47 | −10.14 ± 1.04 | −10.33 ± 0.78 |
| P-value | * | ns | ns |
Data shown are the mean of ± SEM of three replicates. One-way ANOVA with Tukey post-test were performed to measure significance between the MC3R/MC4R expressed alone group (1:0) and the experimental groups. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant.