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. 2022 Feb 17;13:835348. doi: 10.3389/fimmu.2022.835348

Figure 1.

Figure 1

MC vitality and phenotype after the conditioning period. (A) Schematic representation of experimental protocol. BMMC were first stimulated for 24h with LPS, curdlan, IgE/Ag or left untreated (RPMI). Then cells were left to recover for 6 days, countedand tested for viability by means of trypan blue exclusion (B), analyzed for surface marker expression (C), stained with Toluidin blue (D) and Alcian-blu safranin (E) and analyzed for connective and mucosal proteases mRNA expression (F). Data are expressed as mean +SD from n>3 experiments; statistical analysis were performed with one-way Anova with Dunnet correction (**p < 0,01).